Title:
Effect of Temperature Modulation and Different Inoculation Strategies during the SSF of Pretreated Arundo Donax
Author(s):
De Bari, I., Liuzzi, F., Ambrico, A., Trupo, M.
Document(s):
Paper
Abstract:
Simultaneous saccharification and fermentation (SSF) has been considered the most convenient process strategy for the conversion of biomass sugars into bioethanol. During SSF, temperatures typically lower than 40°C favor both hydrolysis and fermentation. However, the latest enzymatic commercial mixtures with improved performances have optimal working temperatures in the range 45-50°C. In order to conjugate this temperature requirement with the optimal conditions for fermentation, temperature modulation during the process could offer the advantage of discontinuously accelerating the hydrolysis while avoiding sugars accumulation. In the present paper, the effect of an intermittent step-wise increase of temperature along with fed-batch inoculation was investigated during the fermentation of Arundo donax carbohydrates to bioethanol. In particular, temperature was periodically raised to 50° to improve the cellulose conversion and the hydrolyzates were inoculated in fed-batch mode each time the temperature diminished to 32°C.The SSF tests were carried out by using products obtained through the Chemtex technologies for biomass pretreatment and enzymatic liquefaction. The performances of several strains belonging to the families of Saccharomyces cerevisiae, and Kluyveromyces marxianus were compared. The results were discussed in terms of ethanol yields, extent of the cellulose digestion, and microrganisms viability through the process. This work is part of the European project Biolyfe.
Keywords:
bioethanol, enzymatic hydrolysis, fermentation, Arundo donax L., demonstration plant
Topic:
R&D on Processes for Solid, Liquid and Gaseous Fuels from Biomass
Subtopic:
Bioethanol production and sugar release from lignocellulosic biomass
Event:
21st European Biomass Conference and Exhibition
Session:
3DV.1.45
Pages:
1558 - 1561
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-3DV.1.45
Price:
FREE
