Title:
Efficient Enzymatic Hydrolysis and Fermentation of Lignocellulosic Biomass at High Solid Loading Conditions
Author(s):
Manzanares, P., Ballesteros, I., Saez, F., Oliva, J.M., Negro, M.J., Ballesteros, M.
Document(s):
Paper
Poster
Abstract:
In this work, the feasibility to produce high ethanol concentrations by enzymatic hydrolysis and fermentation from hydrothermally-pretreated wheat straw has been studied. Wheat straw is an abundant residue from the cereal industry in Europe showing good perspectives for second generation bioethanol production. As residual feedstock, its use in the biomass to ethanol process could contribute to comply with sustainability criteria. Ethanol production from the water insoluble solids (WIS) fraction of hydrothermally-pretreated wheat straw was evaluated in simultaneous saccharification and fermentation (SSF) laboratory experiments at increasing solids up to 20% (w/w). Commercial cellulases and hemicellulases and Saccharomyces cerevisiae as fermenting yeast were employed. Different SSF process conditions such as enzyme and inoculum yeast loading, as well as the effect of a liquefaction step were evaluated. Final ethanol concentration in the fermentation media close to 6% (v/v) were achieved in experiments laboratory flask conditions, corresponding to liquefaction plus SSF (LSSF) yields from 68 to 76% of theoretical. The use of reactor designs such as a rotating drum reactor, showed higher ethanol yields and process performance, opening the possibility to work at initial consistency over 20% total solids.
Keywords:
bioethanol, biological conversion, wheat straw, second generation, yield
Topic:
Biomass Conversion Technologies for Intermediates, Liquid and Gaseous Fuels, Chemicals and Materials
Subtopic:
Bioethanol and sugar release from lignocellulosic biomass
Event:
22nd European Biomass Conference and Exhibition
Session:
3DV.2.32
Pages:
1315 - 1318
ISBN:
978-88-89407-52-3
Paper DOI:
10.5071/22ndEUBCE2014-3DV.2.32
Price:
FREE
