Title:
Molecular Cloning, Bioinformatics and Functional Analysis of JCCBF Gene from Jatropha Curcas L.
Author(s):
Wang, S.S., Wang, H.B., Deng, F.F., Zou, Z.R., Gong, M.
Document(s):
Paper
Poster
Abstract:
The C-repeat binding factors (CBFs)/dehydration-responsive element-binding protein (DREBs) are a group of conserved transcription factors, with fundamental roles in response and adaptation to cold stress in plants, especially chilling-tolerant and herbal species. However, the information on CBFs in chilling-sensitive woody plants is relatively limited. Previously, we performed the global transcriptome sequencing of chilling-treated Jatropha curcas L., an important chilling-sensitive bioenergy woody crop. A novel CBF-like gene, designated JcCBF, was isolated, which contains AP2/EREBPmotifknown to exist in all other plant CBFs. Thephylogenetic analysis for CBF homologs of plants clearly divided data into two groups, and JcCBF was closely related to CBF fromHordeum vulgare.Analysis of its promoter region identified threepossible MYC permutation sequences of theCANNTG core, implying its transcriptional regulation driven by an upstreamfactor termed as the inducer of CBF expression 1 (ICE1). No obvious morphologicdifferences were observed between the WT and the transgenic tobacco with JcCBF over expression, under normal growth conditions, while the transgenic seeds exhibited a higher germination rate and survival ability when grown under a lower temperature (16°C). T2 generation of transgenic tobacco will be subjected to unraveling the molecular mechanism of increased chilling tolerance in future.
Keywords:
Jatropha curcas, molecular cloning, bioinformatics, C-repeat binding factors (CBF), inducer of CBF expression (ICE)
Topic:
Biomass Resources
Subtopic:
Biomass crops and energy grasses
Event:
24th European Biomass Conference and Exhibition
Session:
1DV.1.12
Pages:
309 - 315
ISBN:
978-88-89407-165
Paper DOI:
10.5071/24thEUBCE2016-1DV.1.12
Price:
FREE
