Title:
Kinetic Modelling of the Bioconversion of Corn Cob Waste Biomass by Trained Enterococcus Faecium in a 'One-Pot' System for Succinic Acid Production
Author(s):
Ejekwu, O., Ayeni, A.O., Daramola, M.O.
Document(s):
Paper
Abstract:
An unstructured kinetic model was developed in this study for the one-pot bioconversion of waste corn cob using molten hydrate salt, MHS (ZnCl.4H2O), as the pretreatment solvent and trained Enterococcus Faecium as a biocatalyst with added terms for substrate inhibition. Experimental data were collected from shake flask studies of multiple fermentations at different initial substrate concentrations. The E.faecium cell growth was expressed by an extended Monod model and the Leudeking-Piret model could adequately describe the growth associated production of lactic acid and succinic acid. The model parameters were estimated by minimizing the mean-squared errors between predicted and experimental values and Broyden–Fletcher–Goldfarb–Shanno (BFGS) non-linear optimization code was used. In all cases, the model matched well to the experimental data, this includes the cross-validation experiments performed on the bench top reactor which had a coefficient of determination (R2) value of 0.978.
Keywords:
biorefinery, kinetics, bacteria, corn cob, bioconversion, succinic acid
Topic:
Biomass Conversion to Bio-Based Products and Chemicals
Subtopic:
Processes for bio-based chemicals and materials
Event:
30th European Biomass Conference and Exhibition
Session:
6BO.11.4
Pages:
1042 - 1048
ISBN:
978-88-89407-22-6
Paper DOI:
10.5071/30thEUBCE2022-6BO.11.4
Price:
FREE
