Title:
Lignocellulosic Bioconversion to Microbial Protein via a Cellulolytic Consortium
Author(s):
Peterson, E.C., Hermansen, C., Thong, A., Yong, A., Ho, S., Teo, M., Toledo-Busran, C.
Document(s):
Paper
Slide presentation
Abstract:
Diverse lignocellulosic biomass is globally abundant and is a promising feedstock for microbial bioconversion to food and feed ingredients. However, lignocellulosic biomass is notoriously recalcitrant to bioconversion, and apart from pretreatment also requires sterilization and enzymatic hydrolysis to achieve technical outcomes, which represents bottlenecks to economic feasibility. Consolidated bioprocessing with aerotolerant thermophilic cellulolytic consortia can be applied to overcome these challenges, achieving rapid and complete cellulose conversion into short-chain fatty acids, followed by aerobic fermentation with food-grade microbes. This bioconversion process does not require the addition of enzymes, and generates a consistent mixture of VFAs and ethanol, which can then be fermented into single cell protein and oil. A cocktail of end-products generated rapidly from crystalline cellulose via an anaerobic thermophilic cellulolytic consortium has been confirmed as an excellent substrate for food grade strains Candida utilis, Yarrowia lipolytica, and Corynebacterium glutamicum, generating a microbial biomass yield of 25% from cellulose (dwb.). This microbial biomass is a rich mixture of both protein (42-58%) and lipids (7.8- 8.4%), with high fractions of unsaturated fatty acids, demonstrating this approach holds promise for the bioconversion of lignocellulosic biomass to food and feed ingredients. Such consolidated bioprocessing represents a novel approach for including lignocellulosic residues into the emerging carboxylate platform for value added-products.
Keywords:
cellulose, fermentation, sustainability, residues, food, feeds, consolidated bioprocessing
Topic:
Biomass Conversion to Bio-Based Products and Chemicals
Subtopic:
Processes for bio-based chemicals and materials
Event:
31st European Biomass Conference and Exhibition
Session:
6AO.3.4
Pages:
1012 - 1017
ISBN:
978-88-89407-23-3
Paper DOI:
10.5071/31stEUBCE2023-6AO.3.4
Price:
FREE
