Paper details

Title:

Biotechnological Production of 2-Phenylethanol from Agro-industrial Wastes for Foods and Flavouring Applications

Author(s):

Thimoteo Azevedo Jorge, F., Orynbek, N., Abduraimova, A., Valakhanovich, A., Jacques, P., Besaury, L., Moussa, M., Saulou-Bérion, C., Sánchez-Castañeda, A.K.

Document(s):

Abstract:

This study evaluates the use of apple pomace and whey as low-cost, bio-based substrates for the growth of the yeast strain Kluyveromyces marxianus CBS600, used for sustainable production of a high value aroma compound, 2-phenylethanol (2-PE). Among the waste streams used as carbon and energy source for yeast growth, apple pomace resulted in the highest 2-PE productivity in shake flasks, reaching a concentration of 1.07 g/L in 24 h, while its upscaling to a 1 L bioreactor led to 1.60 g/L in 68 h. In addition, fermentation of acid whey supplemented with yeast extract and minerals for growth purposes significantly improved the bioconversion, leading to 2.64 g/L of 2-PE in 72 h. Using a membrane-based solvent extraction system, 2-PE was efficiently recovered from a defined fermentation medium without impacting cell viability. This method shows promise for extracting 2-PE from waste streams-based fermentation processes. Furthermore, a techno-economic assessment showed that 2-PE production from apple pomace displays a high profitability, with a unit production cost of US$245/kg and an internal rate of return of 13.8%. Hence, this study shows that 2-PE production by fermentation is a sustainable and economically viable process that can contribute to a circular bioeconomy by valorizing agro-industrial residues.

Keywords:

fermentation, techno-economic analysis, 2-phenylethanol, in situ product recovery, agro-industrial wastes

Topic:

Biomass Conversion to Bio-based Products and Chemicals

Subtopic:

Biorefinery platforms for bio-based chemicals and polymers

Event:

32nd European Biomass Conference and Exhibition

Session:

6AO.9.1

Pages:

783 - 785

ISBN:

978-88-89407-24-0

Paper DOI:

10.5071/32ndEUBCE2024-6AO.9.1

Price:

FREE