Title:
Biorefinery Approach to Convert Crude Glycerol to Single Cell Oils (SCOs) and Carotenoids by Rhodosporidium Toruloides DSM70398
Author(s):
Albergo, R., Cirillo, S., Bianco, L., Valerio, V., De Bari, I.
Document(s):
Paper
Poster
Abstract:
In the emerging bioeconomy, the paramount challenge is the use of no inexpensive, cost-effective and sustainable feedstocks. Crude glycerol, a secondary product of the biodiesel industry, fulfils the above features and can be used as a substrate for fermentation without any prior pretreatment. Crude glycerol can be converted by several microorganisms into a range of value-added biochemicals of industrial interest, including organic acids, polyols, microbial lipids (SCOs) and carotenoids. The wild-type yeast Rhodosporidium toruloides DSM70398 can convert crude glycerol to SCOs and carotenoids. The crude glycerol was sourced from a local biodiesel production plant. The fermentation set-up was optimized in flasks using the Response Surface Methodology (RSM). A three-factors with three levels Box-Behnken design was employed to investigate the impact of crude glycerol concentration, C/N molar ratio and temperature on carotenoids and lipids concentration utilizing ammonium sulphate as inexpensive N source. The cellular content of carotenoids and lipids and their final concentration in the fermentation broth were modelled by the experimental design. The maximum concentration of carotenoids predicted was 24.5 ml obtained when C/N=22, glycerol concentration= 49g/l and T=29°C. In this condition, the predicted lipids concentration is 2,28 g/l. A confirmation test was conducted with the above experimental conditions and the carotenoids and lipids concentrations were found to be 26.2±1.5 mg/l and 2.05±0.21 mg/l, respectively. These values were not significantly different from the predicted values according to the t-Student test (p < 0.005, n = 3).
Keywords:
biodiesel, crude glycerol, lipids, carotenoids, circular economy
Topic:
Biomass, Bio-based Products and Bioenergy Integration
Subtopic:
Biomass use in biorefineries
Event:
32nd European Biomass Conference and Exhibition
Session:
3CV.4.12
Pages:
433 - 437
ISBN:
978-88-89407-24-0
Paper DOI:
10.5071/32ndEUBCE2024-3CV.4.12
Price:
FREE
