Title:
Identification of Micrornas Involved in Chilling Response by Deep Sequencing of Jatropha Curcas L. Small Rnas at the Global Genome Level
Author(s):
Wang, S.S., Wang, H.B., Gong, M.
Document(s):
Paper
Poster
Abstract:
Jatropha (Jatropha curcas L.) is becoming one of the most important bioenergy woody plants across the whole world. As a typical chilling-sensitive plants which originated from tropics or subtropics, chilling is considered to be the key environmental factors that limits its growth, production and distribution. Recent studies on several model plants indicate that miRNAs, short, endogenous, non-coding small RNAs, are involved in plant responses to chilling stress. The aim of this study is to identify chilling-responsive conserved and novel miRNAs in J. curcas, at the global genome level, using Illumina Solexa deep sequencing platform. As a result, 788 conserved miRNAs families were found in J. curcas, among which 214 were considered to be chilling-responsive (86 chillinginducd and 128 chilling-repressed). In addition, a total of 196 and 139 novel miRNA precursor candidates were identified from JcCK and JcSam libraries, respectively. After target prediction of both conserved and novel miRNAs, GO enrichment analysis and KEGG pathway annotation were performed to comprehensively describe the property and functions, as well as significantly enriched metabolic/signal pathways in target transcripts. Our results will be helpful for further understanding of involvement of miRNAs in response to chilling stress in J. curcas and chillingsensitive woody plants.
Keywords:
microRNA, chilling, deep sequencing, Jatropha curcas L.
Topic:
Biomass resources
Subtopic:
Energy crops and energy grasses
Event:
21st European Biomass Conference and Exhibition
Session:
1BV.3.27
Pages:
356 - 363
ISBN:
978-88-89407-53-0
Paper DOI:
10.5071/21stEUBCE2013-1BV.3.27
Price:
FREE
